@mhorlbeck used Mann–Whitney U test in https://github.com/mhorlbeck/ScreenProcessing. The t-test approach here was originally extracted from @hanig's notebook in which he processed a V3 screen for his lab.
@AshirBorah has some ideas for assigning empirical p-values relative to negative control guides/oligos, we can discuss it here and implement it moving forward.
Current method:
https://screenpro2.readthedocs.io/en/latest/PhenoScore.html
@mhorlbeck used Mann–Whitney U test in https://github.com/mhorlbeck/ScreenProcessing. The t-test approach here was originally extracted from @hanig's notebook in which he processed a V3 screen for his lab.
@AshirBorah has some ideas for assigning empirical p-values relative to negative control guides/oligos, we can discuss it here and implement it moving forward.