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GoekeLab / m6anet

Detection of m6A from direct RNA-Seq data
https://m6anet.readthedocs.io/
MIT License
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The position output by m6anet seems to be 1 less than the actual position #164

Open wfy1373 opened 1 month ago

wfy1373 commented 1 month ago

Dear m6anet team,

I have encountered an issue while using your tool and analyzing the output data.site_proba.csv file. It appears the transcript_position values reported in the output are off by 1 compared to the actual position of the m6A sites. For example, one row in the data.site_proba.csv file is: ENST00000361453.3,716,28,0.3540786206722260,TGACT,0.2142857142857143 However, the actual position of the A should be 717, not 716.

I wanted to confirm if this observation is correct, is this because m6anet uses 0-based indexing to report the position coordinates?

Thank you in advance for your assistance.

Sincerely, wfy

yuukiiwa commented 1 month ago

Dear @wfy1373,

Yes, m6anet is 0-based, so it is normal for the positions to be off by one.

Thanks!

Best wishes, Yuk Kei