would you recommend setting the "Minimum read counts per gene" argument to something like 10 or 100 for a first try, in order to see real confident positions? My doubt here is that I might lose a lot of sites, since with direct RNA sequencing, I have very low read numbers for most of the genes.
Many thanks for your thoughts.
Best regards, Sophia
Dear m6anet Team,
would you recommend setting the "Minimum read counts per gene" argument to something like 10 or 100 for a first try, in order to see real confident positions? My doubt here is that I might lose a lot of sites, since with direct RNA sequencing, I have very low read numbers for most of the genes.
Many thanks for your thoughts. Best regards, Sophia