Error in EstimateSizeFactorsForMatrix: at least one zero

GoekeLab / proActiv

Estimation of Promoter Activity from RNA-Seq data

https://goekelab.github.io/proActiv/

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Error in EstimateSizeFactorsForMatrix: at least one zero #45

Open notthathime opened 1 year ago

notthathime commented 1 year ago

Thank you very much for sharing your amazing work. I encountered a problem after running Result function.

It showed as below: Calculating junction counts Calculating normalized read counts... converting counts to interger mode Error in EstimateSizeFactorsForMatrix: (counts(object), locfunc = locfunc, : every gene contains at least one zero, cannot compute log geometric means

Could you please help me with this error? What would you suggest to fix this error?

Thank you very much

jleechung commented 9 months ago

This is an error associated with DESeq2's estimateSizeFactors function. Please refer to the discussion here.