Closed daewon-gong closed 11 months ago
Hi @daewon-gong,
Thank you!
Best wishes, Yuk Kei
Hi @yuukiiwa ,
38dbbb5c3531a627c57110ad8c3b628f HEK293T-WT-rep2.tar.gz
94231007837505fddfc374714e112847 HEK293T-Mettl3-KO-rep3.tar.gz
3c85195505b5891752318580e41e28bc 20190918_Sho_M3-2.tar.gz
(This one is the file that seems corrupted in HEK293T-Mettl3-KO-rep3.tar.gz)
data:
KO:
rep1: ./HEK293T_KO_rep1_pre/dataprep
rep2: ./HEK293T_KO_rep2_pre/dataprep
rep3: ./HEK293T_KO_rep3_pre/dataprep
WT:
rep1: ./HEK293T_WT_rep1_pre/dataprep
rep2: ./HEK293T_WT_rep2_pre/dataprep
rep3: ./HEK293T_WT_rep3_pre/dataprep
out: ./out
Thanks for the help!
Best,
Sean Gong
Hi @yuukiiwa I have downloaded the preprocessed HEK293T-Mettl3-KO data and 3 replicates of the HEK293T-WT data from zenodo from your suggested links as given in other discussion threads (https://zenodo.org/record/5103099 and (https://zenodo.org/record/5101752). I unzipped the file using tar -xvzf file.tar.gz command and found all below files for each HEK293T-Mettl3-KO data and HEK293T-Mettl3-WT data -
data.index data.json data.log data.readcount eventalign.index transcript_id_version_merged.gtf
I ran xpore diffmod --config config_file as below
cd $PBS_O_WORKDIR module load xpore/2.1
xpore diffmod --config Hek293T_config2.yml
and obtained an error- "Using the signal of unmodified RNA from /sw/QFAB/miniconda3/envs/xpore_2.1/lib/python3.9/site-packages/xpore/diffmod/model_kmer.csv 0 ids to be testing ..."
My yml file is given below:
data: KO: rep1: /scratch/project_mnt/S0077/xPore/rawdata/HEK293T-METTL3-KO-rep1/new_argsort_dataprep_noNbug rep2: /scratch/project_mnt/S0077/xPore/rawdata/HEK293T-METTL3-KO-rep2/new_argsort_dataprep_noNbug rep3: /scratch/project_mnt/S0077/xPore/rawdata/HEK293T-METTL3-KO-rep3/new_argsort_dataprep_noNbug
WT: rep1: /scratch/project_mnt/S0077/xPore/rawdata/HEK293T-WT-rep1/new_argsort_dataprep rep2: /scratch/project_mnt/S0077/xPore/rawdata/HEK293T-WT-rep2/new_argsort_dataprep rep3: /scratch/project_mnt/S0077/xPore/rawdata/HEK293T-WT-rep3/new_argsort_dataprep
out: /scratch/project_mnt/S0077/xPore/rawdata/diffmod_out method: prefiltering: method: t-test threshold: 0.1
Could you please tell me how to get rid of the problem?
Best regards Reza
Hello,
I noticed some issues while trying to run xpore on 3 replicates of the HEK293T-Mettl3-KO data and 3 replicates of the HEK293T-WT data downloaded from the provided ENA website using aspera.
I get this gzip error while trying to untar
HEK293T-WT-rep2
, the20190918_Sho_M3-2.tar.gz
file in HEK293T-KO-rep3:gzip: 20190918_Sho_M3-2.tar.gz: invalid compressed data--crc error
. I tried re-downloading multiple times and still get the same errors.Giving the preprocessed HEK293T data a try which were downloaded from zenodo. Running
xpore diffmod --config config_file
didn't give any errors but gave empty results with a0 ids to be testing ...
message in the beginning.Could someone check on these files for us. Thank you!
FYI: @jts