GoekeLab / xpore

Identification of differential RNA modifications from nanopore direct RNA sequencing
https://xpore.readthedocs.io/
MIT License
131 stars 23 forks source link

dataprep ERROR #137

Closed zzf77 closed 2 years ago

zzf77 commented 2 years ago

Hi developer, I'm trying to using the practice demo data downloaded from https://zenodo.org/record/5103099/files/demo.tar.gz, and I have successfully installed the latest version of xpore. When I came to the step of dataprep following the help and manual, I kept getting the same ERROR and could not proceed. My working directory: ~/demo/data/HEK293T-METTL3-KO-rep1

Using the command "xpore-dataprep -h" we can see the usage information, which tell us the "summary" is required: (apptest) [zzf@ser1 ~]$ xpore-dataprep -h usage: xpore-dataprep [-h] --eventalign EVENTALIGN --summary SUMMARY --out_dir OUT_DIR [--ensembl ENSEMBL] [--species SPECIES] [--genome] [--n_processes N_PROCESSES] [--readcount_max READCOUNT_MAX] [--resume]

While in https://xpore.readthedocs.io/en/latest/quickstart.html, i don't see the "summary" command.

The running command: xpore-dataprep \ --eventalign /home/dragon/Database/zzf/demo/demo/data/HEK293T-METTL3-KO-rep1/nanopolish/eventalign.txt \ --summary /home/dragon/Database/zzf/demo/demo/data/HEK293T-METTL3-KO-rep1/nanopolish/summary.txt \ --gtf_or_gff /home/dragon/Database/zzf/demo/demo/demo.gtf \ --transcript_fasta /home/dragon/Database/zzf/demo/demo/demo.fa \ --out_dir dataprep \ --genome

And then came this ERROR: xpore-dataprep: error: unrecognized arguments: --gtf_or_gff /home/dragon/Database/zzf/demo/demo/demo.gtf --transcript_fasta /home/dragon/Database/zzf/demo/demo/demo.fa

If i use "xpore dataprep', the tool will come with this: Command 'xpore' not found,did you mean:

It would be grateful if you could give me some advice on solving this problem. Thanks for developing this nice tool.

yuukiiwa commented 2 years ago

Hi @zzf77,

The most updated version of xpore (v2.1) no longer has the --summary command, so it will be great if you can update xpore to v2.1.

Thank you!

Best wishes, Yuk Kei