Can Xpore detect RNA modification other than m6A?

[fulaibaowang]

Hi,

i am wondering how good is xpore detect other RNA modification like ψ and ADP-ribosylation or is it mainly designed for m6A/RNA methylation?

Thanks in advance!

Yun

[yuukiiwa]

Hi Yun (@fulaibaowang),

xpore is a tool for detecting differential modification, so one would have to compare an m6A-writer knockout to a WT to detect the presence of m6A. If you know the conditions of your samples, where a modification is enriched, then you will expect xpore to show the sites with the modification as differentially modified.

Thanks!

Best wishes, Yuk Kei

[fulaibaowang]

Hi YuK,

thanks! I was just wondering if you have tested the performance of xpore on detecting low abundance of modification. m6A is relative abundant but there is other modification that could have lower abundance with which might have problem with false positve calls.