Guppy software version for base calling

GoekeLab / xpore

Identification of differential RNA modifications from nanopore direct RNA sequencing

https://xpore.readthedocs.io/

MIT License

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Guppy software version for base calling #46

Closed mmiladi closed 9 months ago

mmiladi commented 3 years ago

Hello,

Thanks for providing this tool! I was wondering if the version of Guppy can have an impact on the prediction quality and performance. I have data called using Guppy 4.0.9 and 3.2.10. Do they expect to work with the latest version of xpore? Would you recommend to re-base call them with a specific version of Guppy to get a reliable performance of m6A predictions?

Best, Milad

ploy-np commented 3 years ago

Hi @mmiladi,

Sorry for the delayed response. In theory, it shouldn't make much different. In the paper, different Guppy versions were used. However, I recommend to use the same Guppy version across all samples if possible.