I read with great interest your xpore application published in Nat Biotech to evaluate differential RNA modification using nanopore RNA-seq data. I am wondering if we can use xpore to analyze the other RNA-seq data generated by other sequencer platform such as HiSeq 2000 by converting the fastq (acquired from other sequencing platforms) to fast5 files (via other software such as fast5seek;https://github.com/mbhall88/fast5seek)? Or do you have some software we can apply (rather than fast5seek) to do the conversion from fastq to fast5 files for xpore application? Thank you for your support.
Dear Dr. GÖKE:
I read with great interest your xpore application published in Nat Biotech to evaluate differential RNA modification using nanopore RNA-seq data. I am wondering if we can use xpore to analyze the other RNA-seq data generated by other sequencer platform such as HiSeq 2000 by converting the fastq (acquired from other sequencing platforms) to fast5 files (via other software such as fast5seek;https://github.com/mbhall88/fast5seek)? Or do you have some software we can apply (rather than fast5seek) to do the conversion from fastq to fast5 files for xpore application? Thank you for your support.
Yong-Bi Fu