GreenleafLab / ArchR

ArchR : Analysis of Regulatory Chromatin in R (www.ArchRProject.com)
MIT License
388 stars 140 forks source link

How to clusters cells in ArhcR with Signac cluster labels? #1996

Closed momur closed 1 year ago

momur commented 1 year ago

Hello,

I have single cell multiomics data (scRNAseq & scATACseq). I have done preprocessing, quality control and clustering steps in Seurat-Signac. I used Weighted Nearest Neighbor Analysis approach for clustering. The clusters I have are biologically meaningful and I would like to use my barcodes and corresponding cluster labels in my Signac object to annotate cells in ArchR.

I have tried clustering scATACseq data with several different parameters in ArchR but the clusters dont look similar to what I got. I have tried labelling scATAC-seq clusters with scRNA-seq information following ArchR tutorial but since clustering step in ArchR doesn't result in similar clusters, label transfer was not successful.

Barcodes are matching. So I believe that there should be way to use barcodes and cluster labels from Signac to annotate cells in ArchR. I would like to know that how can I do it in ArchR?

Thanks!

rcorces commented 1 year ago

Hi @momur! Thanks for using ArchR! Please make sure that your post belongs in the Issues section. Only bugs and error reports belong in the Issues section. Usage questions and feature requests should be posted in the Discussions section, not in Issues.
It is worth noting that there are very few actual bugs in ArchR. If you are getting an error, it is probably something specific to your dataset, usage, or computational environment, all of which are extremely challenging to troubleshoot. As such, we require reproducible examples (preferably using the tutorial dataset) from users who want assistance. If you cannot reproduce your error, we will not be able to help. Before going through the work of making a reproducible example, search the previous Issues, Discussions, function definitions, or the ArchR manual and you will likely find the answers you are looking for. If your post does not contain a reproducible example, it is unlikely to receive a response.
In addition to a reproducible example, you must do the following things before we help you, unless your original post already contained this information: 1. If you've encountered an error, have you already searched previous Issues to make sure that this hasn't already been solved? 2. Did you post your log file? If not, add it now. 3.__ Remove any screenshots that contain text and instead copy and paste the text using markdown's codeblock syntax (three consecutive backticks). You can do this by editing your original post.