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GuckLab / impose

Align microscopy images using custom-defined structural patterns and superimpose those on data from other imaging modalities.
GNU General Public License v3.0
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Cannot open multiple files in 'Colocalize' #50

Closed Mahajan30 closed 2 years ago

Mahajan30 commented 2 years ago

I am able to open multiple files in 'Collect' and put masks/structures on them, but unable to open (same) multiple files in 'Colocalize'. The error I get is this:

Unhandled exception in Impose version 0.3.1: Traceback (most recent call last): File "/Volumes/Impose_0.3.1/Impose.app/Contents/MacOS/impose/gui/colocalize.py", line 184, in on_add_data self.add_paths(paths) File "/Volumes/Impose_0.3.1/Impose.app/Contents/MacOS/impose/gui/colocalize.py", line 109, in add_paths self.session_scheme.append(pp) File "impose/session.py", line 157, in append structure_composite = self.scs.get_mean() File "impose/structure/composite_stack.py", line 80, in get_mean raise NotImplementedError("Averaging not implemented yet!") NotImplementedError: Averaging not implemented yet!

Could you please look into this? Currently I have to close Impose after analysing each measurement. Resolving this would make life easy :)

paulmueller commented 2 years ago

The idea behind all of this is that if you load multiple structures, you can compute an average structure which you can then overlay with other data. Since this averaging feature (#4) is not implemented, you get a NotImplementedError.

What (I think) you would like to do does not align with the working principle of impose. You always can only analyze one dataset in one session. What could resolve your problem is to use "Clear Session" in the file menu. Then you don't have to restart impose anymore.

Mahajan30 commented 2 years ago

Okay understood! Thanks!