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GuyTeichman / RNAlysis

Analyze your RNA sequencing data without writing a single line of code
https://guyteichman.github.io/RNAlysis/
MIT License
129 stars 13 forks source link

Unable to run RNAlysis #15

Closed Rajeshwarira closed 1 year ago

Rajeshwarira commented 1 year ago

Unable to run RNAlysis I tried to run RNAlysis with the data given in the example part of user guide(analysis 1) as a sample run since I'm using this for first time. I was not able run it and getting the following errror which I attached as photo. Please do help me troubleshooting this. Device version: Windows 10 IMG20230406160416

GuyTeichman commented 1 year ago

Hi Rajeshwarira, It looks like the file you're trying to open is a Microsoft Excel file (.xlsx), and not a csv file, and this is the source of the issue.

I would suggest that you open the file with a program like Excel, and then click on "File" - >"Save As", and change the format through the "Save As Type" drop-down menu to "CSV (Comma Delimited)".

See the link below for more details: https://www.google.com/url?sa=t&source=web&rct=j&url=https://support.microsoft.com/en-us/office/import-or-export-text-txt-or-csv-files-5250ac4c-663c-47ce-937b-339e391393ba%23:~:text%3DYou%2520can%2520convert%2520an%2520Excel,or%2520CSV%2520(Comma%2520delimited).&ved=2ahUKEwi6oq_3qNv7AhULi_0HHduqCr8QFnoECA0QBQ&usg=AOvVaw0eUOr90h9lvor4Q0b0nw0n

Feel free post another comment here if you're still having trouble loading the file. Good luck with your analysis!

Rajeshwarira commented 1 year ago

Thank you so much😊. I'll try it and let you know how it goes.

On Sat, Apr 8, 2023, 3:12 PM Guy Teichman @.***> wrote:

Hi Rajeshwarira, It looks like the file you're trying to open is a Microsoft Excel file (.xlsx), and not a csv file, and this is the source of the issue.

I would suggest that you open the file with a program like Excel, and then click on "File" - >"Save As", and change the format through the "Save As Type" drop-down menu to "CSV (Comma Delimited)".

See the link below for more details:

https://www.google.com/url?sa=t&source=web&rct=j&url=https://support.microsoft.com/en-us/office/import-or-export-text-txt-or-csv-files-5250ac4c-663c-47ce-937b-339e391393ba%23:~:text%3DYou%2520can%2520convert%2520an%2520Excel,or%2520CSV%2520(Comma%2520delimited).&ved=2ahUKEwi6oq_3qNv7AhULi_0HHduqCr8QFnoECA0QBQ&usg=AOvVaw0eUOr90h9lvor4Q0b0nw0n

Feel free post another comment here if you're still having trouble loading the file. Good luck with your analysis!

— Reply to this email directly, view it on GitHub https://github.com/GuyTeichman/RNAlysis/issues/15#issuecomment-1500847944, or unsubscribe https://github.com/notifications/unsubscribe-auth/A6TURHPKT6OWYRMVADNT3DTXAEXHLANCNFSM6AAAAAAWXH52VQ . You are receiving this because you authored the thread.Message ID: @.***>

Rajeshwarira commented 1 year ago

Thank you for the response it worked well. I need help in installing kallisto. I tried to add path using command prompt and the following appeared. Please do tell me how should I install and run kallisto. IMG20230413122259

GuyTeichman commented 1 year ago

Hi, did you download and unzip kallisto from their website?

Rajeshwarira commented 1 year ago

Yes, I did.

On Thu, Apr 13, 2023, 1:44 PM Guy Teichman @.***> wrote:

Hi, did you download and unzip kallisto from their website?

— Reply to this email directly, view it on GitHub https://github.com/GuyTeichman/RNAlysis/issues/15#issuecomment-1506542566, or unsubscribe https://github.com/notifications/unsubscribe-auth/A6TURHJNAS4ZLBMYOXX7MT3XA6YXZANCNFSM6AAAAAAWXH52VQ . You are receiving this because you modified the open/close state.Message ID: @.***>

GuyTeichman commented 1 year ago

Hi, You can either try using the following guide to add the kallisto folder to your PATH: https://www.architectryan.com/2018/03/17/add-to-the-path-on-windows-10/

Or when running kallisto through RNAlysis, set the 'kallisto_installation_folder' parameter to point to your kallisto installation folder.

Let me know if that works for you.

Rajeshwarira commented 1 year ago

Okay I'll try and let you know ☺️ thank you for your support.

On Thu, Apr 13, 2023, 2:54 PM Guy Teichman @.***> wrote:

Hi, You can either try using the following guide to add the kallisto folder to your PATH: https://www.architectryan.com/2018/03/17/add-to-the-path-on-windows-10/

Or when running kallisto through RNAlysis, set the 'kallisto_installation_folder' parameter to point to your kallisto installation folder.

Let me know if that works for you.

— Reply to this email directly, view it on GitHub https://github.com/GuyTeichman/RNAlysis/issues/15#issuecomment-1506639683, or unsubscribe https://github.com/notifications/unsubscribe-auth/A6TURHNT5WKJ27QA2K7IUXTXA7A4FANCNFSM6AAAAAAWXH52VQ . You are receiving this because you modified the open/close state.Message ID: @.***>

Rajeshwarira commented 1 year ago

It worked ☺️Thank you so much for your support.

On Thu, Apr 13, 2023, 3:08 PM rajarajeshwari rajkumar < @.***> wrote:

Okay I'll try and let you know ☺️ thank you for your support.

On Thu, Apr 13, 2023, 2:54 PM Guy Teichman @.***> wrote:

Hi, You can either try using the following guide to add the kallisto folder to your PATH: https://www.architectryan.com/2018/03/17/add-to-the-path-on-windows-10/

Or when running kallisto through RNAlysis, set the 'kallisto_installation_folder' parameter to point to your kallisto installation folder.

Let me know if that works for you.

— Reply to this email directly, view it on GitHub https://github.com/GuyTeichman/RNAlysis/issues/15#issuecomment-1506639683, or unsubscribe https://github.com/notifications/unsubscribe-auth/A6TURHNT5WKJ27QA2K7IUXTXA7A4FANCNFSM6AAAAAAWXH52VQ . You are receiving this because you modified the open/close state.Message ID: @.***>