CROPSR is a python tool designed for genome-wide gRNA design and evaluation for CRISPR experiments, with special focus on complex genomes such as those found in energy-producing crops. CROPSR is a product of the DOE Center for Advanced Bioenergy and Bioproducts Innovation (CABBI).
Hello dear.
I am trying to obtain the primer list of a gene using the prmrdsgn2 module. However, I only have the bowtie2 alignment result and not the output file which contains this information: Primer ID, Primer Sequence, ,5' Position ,Amplicon Size, Alignment Score.
This is the command line:
python3 prmrdsgn2.py -i data/gene.fasta -g ../data/genome.fasta -o data/primers.fasta -v -F.
Could you tell me what the error is.
Note: Reviewing the script the module has two functions create_sam_dataframe() parse_sam_file() that apparently have the function to create and generate the output file.
Hello dear. I am trying to obtain the primer list of a gene using the prmrdsgn2 module. However, I only have the bowtie2 alignment result and not the output file which contains this information: Primer ID, Primer Sequence, ,5' Position ,Amplicon Size, Alignment Score. This is the command line: python3 prmrdsgn2.py -i data/gene.fasta -g ../data/genome.fasta -o data/primers.fasta -v -F. Could you tell me what the error is.
Note: Reviewing the script the module has two functions create_sam_dataframe() parse_sam_file() that apparently have the function to create and generate the output file.
Regards
Jean Pierre Ramos