No such file or directory when doing miRNA analysis

[Ben7124]

Hello,

When running the miRNA module with the command: XICRA miRNA --input datafolder --software miraligner optimir sRNAbench I get the following error:

Can you please help me troubleshoot the issue? Thank you!

--------- Starting Process --------- 09/19/2022, 09:22:31

• Software for miRNA analysis selected: ['miraligner', 'optimir', 'sRNAbench']

• Getting files from input folder...

• Mode: join.

• Extension: [_joined.fastq]

• Input folder exists 12 files selected... 12 samples selected... Single end mode selected...

• Species provided: hsa

• Create folder to store results: /home/ubu/miniconda3/envs/XICRA/lib/python3.7/site-packages/XICRA/modules/db_files

  • miRNA gff file available
  • hairpin fasta file available
  • miRNA gff file available
  • miRBase str file available

• Create a miRNA analysis for each sample retrieved... ***ERROR: <Future at 0x7fe75ee55550 state=finished raised FileNotFoundError> 'CONTROL_1' generated an exception: [Errno 2] No such file or directory: '/home/ubu/datafolder/data/CONTROL_1/miRNA/miraligner/CONTROL_1-tab.freq.txt'

same error here for all samples......

• miRNA analysis is finished...
• Let's summarize all results...
• Input folder exists

**ERROR: No samples were retrieved for this option. Continue processing...

[Ben7124]

Works now!

[JFsanchezherrero]

Hi there, Happy to hear it worked.

thanks for the feedback! Best regards