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HRGV / phyloFlash

phyloFlash - A pipeline to rapidly reconstruct the SSU rRNAs and explore phylogenetic composition of an illumina (meta)genomic dataset.
GNU General Public License v3.0
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Phyloflash refernce database aborts during bowtie build #166

Open whitnersyrena opened 2 years ago

whitnersyrena commented 2 years ago

Hi there,

I'm trying to run phyloflash on a MacOS and having an issue while trying to create the initial database. It gets through to the bowtie build step and then gives the following error:

running subcommand: /opt/anaconda3/envs/pf/bin/bowtie-build ./138.1/SILVA_SSU.noLSU.masked.trimmed.NR96.fixed.fasta ./138.1/SILVA_SSU.noLSU.masked.trimmed.NR96.fixed.bt -q 2>tmp.bowtiebuild.log sh: line 1: 78886 Abort trap: 6 /opt/anaconda3/envs/pf/bin/bowtie-build ./138.1/SILVA_SSU.noLSU.masked.trimmed.NR96.fixed.fasta ./138.1/SILVA_SSU.noLSU.masked.trimmed.NR96.fixed.bt -q 2> tmp.bowtiebuild.log [14:57:38] FATAL: Tool execution failed!. Error was 'No such file or directory' and return code '34304' Check error log file tmp.bowtiebuild.log Aborting. [14:57:38] Saving log to file phyloFlash_log_on_error

Here is the information provided in the bowtiebuild.log file: dyld: Library not loaded: @rpath/libtbb.dylib Referenced from: /opt/anaconda3/envs/pf/bin/bowtie-build-s Reason: image not found

If anybody could please advise on how to solve this issue that would be GREATLY appreciated :)

Thanks, Syrena