New term: protease accessibility laddering

HUPO-PSI / psi-mi-CV

Molecular Interactions Controll Vocabulary

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New term: protease accessibility laddering #103

Closed noedelta closed 8 years ago

noedelta commented 18 years ago

As described in PMID:16615907, in protease accessibility laddering (PAL) tagged proteins are purified on magnetic beads in their natively folded state. While attached to the beads, proteins are probed with proteases. Proteolytic fragments are eluted and detected by immunoblotting with antibodies against the tag (e.g., Protein A, GFP, and 6xHis).

The term should be most likely a child of footprinting (MI:0417)

Reported by: lukasz99

noedelta commented 18 years ago

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Please, note that this term could be also made a child of protein footprinting (MI:0436) which in turn can be mae a child of both feature detection and interaction detection methods

Original comment by: lukasz99

noedelta commented 18 years ago

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I agree to both new term and extension to feature detection

Original comment by: luisa_montecchi

noedelta commented 18 years ago

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Accepted at the PSI Washington meeting added as MI:0813

Original comment by: luisa_montecchi

noedelta commented 17 years ago

status: open --> closed

Original comment by: luisa_montecchi