search

HUPO-PSI / psi-mi-CV

Molecular Interactions Controll Vocabulary
Creative Commons Attribution 4.0 International
4 stars 4 forks source link

immunodepletion analysis #124

Closed noedelta closed 8 years ago

noedelta commented 17 years ago

The use of antibodies raised against one protein to detect the depletion of the complex of interacting proteins. The depletion of the complex is indicates the interacting proteins.

Reported by: jyotikhadake

noedelta commented 17 years ago

Logged In: YES user_id=653048 Originator: NO

Do you mean 'antibody blockade'? This method cannot be used to detect new interactions but to study the effets of a known interaction disruption. So I am a bit reluctant to put is as an interaction method as it is a 'negative interaction observation'. Could you give a reference?

Original comment by: luisa_montecchi

noedelta commented 17 years ago

Logged In: YES user_id=653048 Originator: NO

Follonwing discussion immunodepletion will be a child of CoIP and defined as 'Method involving 2 CoIp on the same sample, a control one where an interaction is detected, and a second one where the sample is previously treated with a specific antibody that precipitate a candidate interactor and lead to an interaction supression.' example in 11980720: interaction between baz1a and baz1b 17190598: interaction between atxn1 and cic 17081976: interaction between BRCA1 and BARD1 9792637:

Original comment by: luisa_montecchi

noedelta commented 17 years ago

Logged In: YES user_id=653048 Originator: NO

see MI:0858

Original comment by: luisa_montecchi

noedelta commented 17 years ago

Original comment by: luisa_montecchi