Interaction detection method: Filter retardation assay

[noedelta]

Some people seem to filter their sample through a filter and then test if their protein got retained on it. If yes they infer the protein forms large aggregetes - this seems to be used to test primarily for amyloid formation - vide eg PMID:10859365 or PMID:18434538 Is this a valid method of interaction detection ? If yes a new term is needed (child of biochemical, I'd guess)

lukasz

Reported by: lukasz99

[noedelta]

Logged In: YES user_id=653048 Originator: NO

kind of a molecular sieving not on Gel but on a filter? shall this be child of chromatography technology MI:0091? they call it Filter-trap assay I quite like the name as there is not much retardation but rather retention. tentative definition: Purified protein are mixed in a buffer and the resulting mixture is filtered through a cellulose-acetate filters (0.2 μm pore size). Big aggregates are retained on the filter and later characterized by antibody.

see MI:0928

Original comment by: luisa_montecchi

[noedelta]

• status: open --> closed

Original comment by: luisa_montecchi

[noedelta]

Logged In: YES user_id=719654 Originator: YES

>kind of a molecular sieving not on Gel but on a filter?

not exactly - molecular sieving happens when you go inside beads and slow down. here you just can't squeeze through a pore...

a variation of this is to run apge and look for things retained without even entering the stacking gel... at least this is what some amyloid people do...

>shall this be child of chromatography technology MI:0091?

I would keep it directly as a biochemical method - I'm not sure if chromatography people would like a mambrane pretending it'sa column ;o) maybe we can wait until more assays like that pop up to see how to group them ?

luaksz

Original comment by: lukasz99