HUPO-PSI / psi-mi-CV

Molecular Interactions Controll Vocabulary
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filter retardation assay #184

Closed noedelta closed 8 years ago

noedelta commented 15 years ago

this one is for PMID:18434538 (fig 3H) - protein aggregation is monitored by retention of the aggregates by a porous membrane. I would guess it should be a child of biochemical method as molecular sieving/gel filtration principle seems to be quite different.

lukasz

Reported by: lukasz99

noedelta commented 15 years ago

is this not MI:0928 Name: filter trap assay

though the definition needs rewording. For example, I'll delete the bit about subsequent Ab detection since that adds a prerequisite interaction detection method which is too limiting. Also I don't see that the filter type should be specified as glass fibre filters have been used for this for years.

Sandra

Original comment by: orchard

noedelta commented 15 years ago

yes indeed ;o) sounds like filter trap (minus antibody reference which should go away) lukasz

Original comment by: lukasz99

noedelta commented 15 years ago

Updated as discussed.

Original comment by: orchard

noedelta commented 15 years ago

Original comment by: orchard