noedelta
commented
15 years ago New child of ‘Biophysical’ (MI:0013) ‘Detection by mass spectrometry’, definition 'Study of interactions by an analytical technique based on measurements of mass-to-charge ratio of charged particles in a mass spectrometer'. Existing term ‘mass spectrometry studies of complexes’ (MI:0069) and new term 'mass spectrometry study of hydrogen/deuterium exchange' will be children of that term. Definition of MI0069 will be changed to be less protein-centric and encompass all macromolecules. Existing Participant Detection method ‘mass spectrometry’ will be renamed ‘Identification by mass spectrometry’ to differentiate between the two terms.
Original comment by: orchard
current definition of MI:0069: mass spectrometry studies of complexes: 'Mass spectrometric approaches to the study of protein in complexes permits the identification of subunit stoichiometry and transient associations. By preserving complexes intact in the mass spectrometer, mass measurement can be used for monitoring changes in different experimental conditions, or to investigate how variations of collision energy affect their dissociation.'
seems to be to specific as it does not encompass, for example, experiments where H/D-exchange is monitored by mass spec; observed changes are used to infer interactions between monitored molecule (eg protein) and whatever other molecule was added (in principle can be anything - protein, nucleic acid, cofactor). See, eg PMID: 18948593
Therefore I would suggest:
Reported by: lukasz99