PING interaction detection method

[noedelta]

In Vitro method described in:

An in vitro microfluidic approach to generating protein-interaction networks.

Gerber D, Maerkl SJ, Quake SR. Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, 318 Campus Drive, Stanford, CA 94305, USA. We developed an in vitro protein expression and interaction analysis platform based on a highly parallel and sensitive microfluidic affinity assay, and used it for 14,792 on-chip experiments, which exhaustively measured the protein-protein interactions of 43 Streptococcus pneumoniae proteins in quadruplicate. The resulting network of 157 interactions was denser than expected based on known networks. Analysis of the network revealed previously undescribed physical interactions among members of some biochemical pathways. PMID: 19098921

Reported by: jyotikhadake

[noedelta]

Not sure this really is a novel interaction detection method, whatever the authors claim - its an in situ preparation of a protein array chip. If it is accepted as a new method, however, I would suggest child of protein array.

Snadra

Original comment by: orchard

[noedelta]

I'd guess a child of protein array would be fine... lukasz

Original comment by: lukasz99

[noedelta]

MI:02174

Original comment by: orchard

[noedelta]

• status: open --> closed

Original comment by: orchard