noedelta
commented
13 years ago I think this one can be annotated as a normal CH-Ip rather than creating a new term "Centromeric minichromosomes containing LacO repeats are captured by LacI-Flag affinity purification."
Original comment by: orchard
Mini-chromosomes that contain sequences of interest are flanked with repetitive Lac operator sequences. The mini-chromosomes are then selectively purified from the bulk of chromatin using an immobilized Lac repressor. The associated complexes are identified by LC MS/MS Akiyoshi, B., Nelson, C. R., Ranish, J. A. & Biggins, S. (2009) Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit. Genes Dev 23, 2887-2899. 35 Unnikrishnan, A., Gafken, P. R. & Tsukiyama, T. (2010) Dynamic changes in histone acetylation regulate origins of DNA replication. Nat Struct Mol Biol 17, 430-437.
Reported by: jyotikhadake