Closed noedelta closed 8 years ago
An alternative (worse, IMO) is to leave BifFC, as it is now, specific to YFP and localization but modify MI:0229 (GFP complementation) name/definition to allow for different variants of reconstituted fluorophores there.
Original comment by: lukasz99
Terms merged with updated definition
Original comment by: orchard
Original comment by: orchard
Definition of the term is too specific - it explicitly refers to YFP, whereas other GFP derivatives/homologs can be also used. Proposed updated version:
The bimolecular fluorescence complementation (BiFC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two non fluorescent fragments of a protein fluorophore such as green fluorescent protein (GFP) or its derivatives. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional flourophore that can be identified through fluorescence spectroscopy or microscopy.
Reported by: lukasz99