search

HUPO-PSI / psi-mi-CV

Molecular Interactions Controll Vocabulary
Creative Commons Attribution 4.0 International
4 stars 4 forks source link

venus-c and -c; firefly luciferase complementation assay #287

Closed noedelta closed 8 years ago

noedelta commented 12 years ago

Hi,

I was reviewing PMID:22269274 and there different types of complementation assays are used. For starters, a venus fluorescent protein complementation assay is used, so I found appropiate to create these two new feature types for the c- and n-terminal venus fragments tags that are used in that assay:
venus-c IA:2052 venus-n IA:2053 These two terms have been created internally for IntAct and need to be released to the MI controlled vocabulary. Apart from that, in the same publication, a split firefly luciferase complementation assay is used. We only have in the moment a Split renilla luciferase complementation (MI:1037) as interaction detection method and no feature tags for the c- and n-terminal fragments. I suggest that we create the following terms: 1.- "split luciferase complementation assay" to substitute MI:1037 and allow using it with both renilla and firefly tags. The old definition needs updating: OLD "The N-terminal portion of synthetic renilla luciferase (hrluc) is attached to one protein through the linker peptide (GGGS)2 and C-terminal portion of synthetic renilla luciferase is connected to the second protein through the linker (GGGGS)2. Interaction of the 2 proteins recovers hrluc activity and produces light." PROPOSED NEW: "The N-terminal portion of a synthetic luciferase is attached to one protein and the C-terminal portion of the same synthetic luciferase is connected to the second protein. Interaction of the 2 proteins recovers the luciferase activity and produces light." 2.- "renilla-c" as "C-terminal fragment of renilla luciferase". Child of "renilla luciferase protein tag" (MI:0896). 3.- "renilla-n" as "N-terminal fragment of renilla luciferase". Child of "renilla luciferase protein tag" (MI:0896). 4.- "firefly luciferase protein tag", child of "fusion protein" (MI:0240). 5.- "firefly-c" as "C-terminal fragment of firefly luciferase". Child of "firefly luciferase protein tag". 6.- "firefly-n" as "N-terminal fragment of firefly luciferase". Child of "firefly luciferase protein tag".

Best regards,

Pablo.

Reported by: pporras

noedelta commented 12 years ago

Please refer to tracker ID: 3510020 by jyoti

Original comment by: jyotikhadake

noedelta commented 12 years ago

All done.

Original comment by: orchard

noedelta commented 12 years ago

Original comment by: orchard