Are EMSA and Far WB children of solid state assay

[noedelta]

I just noticed that EMSA and far Western blot

are children of 'solid phase assay' but to not comply with the definition of binding to a solid surface. Shouldn't they be 'comigration in gel electrophoresis' (which EMSA is ALSO a child of)?

Reported by: bmeldal

[noedelta]

I'm not sure why EMSA ended up as a solid phase assay - it looks to me as a variant of comigration in non-denaturing gel...

Far Western as a solid phase assay seems to be fine - at least as long as it is used to describe a conventional far western experiment in which proteins are initially separated in gel electrophoresis and transferred to a membrane; to test if a probe protein (prey) binds to one of them it is bound to the membrane (solid phase) and visualized (autorad, antibody, or like). The initially separated proteins transferred to the membrane act as baits.

Original comment by: lukasz99

[noedelta]

Yes, you are right. Far WB can be solid phase assay as the actual interaction is observed during the filter binding step!

Original comment by: bmeldal

[noedelta]

Cleaned up

Original comment by: orchard

[noedelta]

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Original comment by: orchard