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HUPO-PSI / psi-mi-CV

Molecular Interactions Controll Vocabulary
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uracil interference assay - new cv term #367

Closed noedelta closed 8 years ago

noedelta commented 9 years ago

def: "In uracil interference assays, the DNA will be amplified by PCR in the presence of a mixture of TTP and dUTP to randomly replace thymine by deoxyuracil residues before the binding assay. If T nucleotides involved in protein-DNA interactions are replaced by deoxyuracil, protein binding is prevented. After isolating the protein-DNA complex, DNA is cleaved with uracil-N-glycosylase, which specifically targets uracil bases, and the products are electrophoresed on a denaturing polyacrylamide gel. As a result, DNA in which a thymine involved in binding is replaced by uracil will be depleted. Hence, although the pattern looks like a footprint, the blank region means \"contact points\"." [PMID:18265086] is_a: MI:0605 ! enzymatic footprinting

Reported by: kroey

noedelta commented 9 years ago

Original comment by: orchard