search

HUPO-PSI / psi-mi-CV

Molecular Interactions Controll Vocabulary
Creative Commons Attribution 4.0 International
4 stars 4 forks source link

CLASH #379

Closed noedelta closed 8 years ago

noedelta commented 9 years ago

It is a method to identify RNA-RNA interactions that occur in the proximity of an RNA binding protein of interest. The RNA is crosslinked to the protein, that is immunoprecipitated. After RNAsi treatment the RNA hybrids protected from degradation by the protein are ligated and chimeras are sequenced.

Reference Grzegorz Kudla1,2, Sander Granneman1, Daniela Hahn1, Jean D. Beggs, and David Tollervey. Cross-linking, ligation, and sequencing of hybrids reveals RNA–RNA interactions in yeast Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):10010-5. PMID: 21610164

Reported by: simonapanni

noedelta commented 9 years ago

This issue handled with the batch of terms created in issue #379.

Original comment by: pporras

noedelta commented 9 years ago

Original comment by: pporras