SRET:Sequential BRET-FRET;BRET combined with FRET. Oxidation of a Renilla Luciferase substrate triggers acceptor excitation by BRET and subsequent energy transfer to a FRET acceptor. YFP emission was determined after adding DeepBlueC as luciferase substrate.
From example in PMID:27129257
SRET:Sequential BRET-FRET;BRET combined with FRET. Oxidation of a Renilla Luciferase substrate triggers acceptor excitation by BRET and subsequent energy transfer to a FRET acceptor. YFP emission was determined after adding DeepBlueC as luciferase substrate.