pporrasebi
commented
3 years ago It is not clear to me whether the tag used in PMID:32150576 is the full length GCN4 or the peptide described in the paper you mention. Also, from reading such paper I interpret there could be three potential tags:
- GCN4 as a tag, possibly (but I think not likely) full length.
- GCN4 peptide, potentially even single repeat, but more likely multiple repeats (low copy number).
- Sun Tag: 24 repeats of peptide GCN4 sequence, used to amplify GFP signal in combination with anti-GCN4, GFP-tagged antibodies
Or if these distinctions are deemed not important for the particular use case you have, I can create a generic GNC4 tag and then add specifics if needed. I'd like to avoid creating tags that are used in just one publication, hence the question.
GCN4 is antibody recognizing GCN4pep peptide. The paper
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252608/
uses GCN4pep to tag proteins and GCN4-GFP fusion to detect it.
GCN4 seems to be also used on its own as a tag: See PMID: 32150576