MUDPIT

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Multidimensional protein identification technology (MUDPIT). MudPIT uses two chromatography steps interfaced back to back in a fused silica capillary. The advantage of this is that the band broadening associated with many chromatographic steps (which can lead to loss of resolution, and hence components running into one another) is avoided and also the capillary can be placed directly into the ion source of a mass spectrometer maximising sensitivity. Chromatography proceeds in steps with increases in salt concentration used to free peptides from the cation-exchange resin after which they bind to a reversed phase resin. A typical reversed phase gradient to increasing hydrophobicity is then applied to progressively elute peptides from the reversed phase packing into the mass spectrometer. Typically this mass spectrometer will be an tandem electrospray, so peptides are ionised in the liquid phase, separated in a primary mass spectrometer, broken up using collision induced dissociation and analysed again.

References: Eng JK, McCormack AL, and Yates JR 3rd, An Approach to Correlate Tandem Mass Spectral Data of Peptides with Amino Acid Sequences in a Protein Database. J Am Soc Mass Spectrom 1994, 5: 976-989

Link AJ, Eng J, Schieltz DM, Carmack E, Mize GJ, Morris DR, Garvik BM, Yates JR 3rd. Direct analysis of protein complexes using mass spectrometry. Nat Biotechnol. 1999;17(7):676-82

Washburn MP, Wolters D, Yates JR 3rd. Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat Biotechnol. 2001;19(3):242-7

Lin D, Alpert AJ, and Yates JR 3rd, Multidimensional Protein Identification Technology as an Effective Tool for Proteomics. American Genomic/Proteomic Technology, 2001 1(1): 38-46

Reported by: jyotikhadake

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Logged In: YES user_id=653048

Is it fine if this term go under participant identification method as a child of mass spec methods?

Original comment by: luisa_montecchi

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added Mudpit MI:0658

Original comment by: luisa_montecchi

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• status: open --> closed

Original comment by: luisa_montecchi