Update of methods to become less protein-centric

[noedelta]

Many of the methods could become more generic just by a slight rewriting of the description to encompass nucleic acids and also small molecules. The example I came across was "filter binding" - from the description I couldn't use it to describe a protein-nucleic acid interaction but in fact there was no change to the technique. Similarly, I used filter binding methods for many years to separate radiolabelled proteins from 32P-ATP which could be used to demonstrate a protein:small molecule interaction. We need to go through all the methods to highlight those which could be made more generic and create new children if necessary.

Sandra

Reported by: orchard

[noedelta]

Logged In: YES user_id=653048

Absolutely right. A list of terms definitions to revisit would help a lot.

Original comment by: luisa_montecchi

[noedelta]

Logged In: YES user_id=653048

In the case of filter binding the current definition is : Proteins expressed by different clones of an expression library are bound to a nitrocellulose membrane, by colony (bacterial library) or plaque (phage library) blotting. A labelled protein can then be used as a probe to identify clones expressing proteins that interact with the probe. Interactions occur on nitrocellulose filters. The method is highly general and therefore widely applicable, in that proteins as diverse as transcription factors and growth factor receptors have been successfully used as probes. A variety of approaches can be used to label the protein ligand, alternatively the ligand can be detected by a specific antibody.

and could become : A method in which separation depends upon the ability of one participant to bind to a filter or membrane which the other participants do not. Molecules interacting with the bound molecule will also be retain on the filter. For example, proteins expressed by different clones of an expression library are bound to a nitrocellulose membrane, by colony (bacterial library) or plaque (phage library) blotting. A labelled protein can then be used as a probe to identify clones expressing proteins that interact with the probe. Interactions occur on the nitrocellulose filters. The method is highly general and therefore widely applicable. A variety of approaches can be used to label the ligand, alternatively the ligand can be detected by a specific antibody.

If think it is better not splitting the term into protein filter binding and filter binding to avoid graph explosion of the CV interaction detection method, and also because it would be redundant with the interactor type.

So I propose to only update the definition of filer binging (MI:0049).

Original comment by: luisa_montecchi

[noedelta]

• status: open --> closed

Original comment by: luisa_montecchi