HUPO-PSI / psi-mi-CV

Molecular Interactions Controll Vocabulary
Creative Commons Attribution 4.0 International
4 stars 4 forks source link

yeast two hybrid-specific features #80

Closed noedelta closed 8 years ago

noedelta commented 18 years ago

there's currently no way to specify the domains used to prepare fusions used in yeast two hybrid experiments. the minimum set seems to be:

- DNA-binding domain fusion

terms, both added as children of 'fusion protein(s)' with specific domain types further added as children of these two

Reported by: lukasz99

noedelta commented 18 years ago

Logged In: YES user_id=653048

Fine for me in principle but is that not implicit from the bait and prey experimental role? I am afraid of redundancy.

Original comment by: luisa_montecchi

noedelta commented 18 years ago

Logged In: YES user_id=719654

True.Except there are different activation domains and different DNA-binding domains to be used. Plus, in principle, one can attach each one on either N or C terminus.

Original comment by: lukasz99

noedelta commented 18 years ago

Logged In: YES user_id=653048

SA PSI meeting:

Original comment by: luisa_montecchi

noedelta commented 18 years ago

Original comment by: luisa_montecchi