New genetic experimental form branch? (BioPAX)

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(see issue 1500524 for more background)

BioPAX needs a place to store terms related to the experimental form of a gene in a genetic interaction experiment.

knock-out: The gene has been completely removed e.g. by genetic engineering knock-down''': The gene expression has been significantly reduced by introduction of an external substance, e.g. by RNA interference hypomorph''': The gene function has been significantly reduced by altering its sequence e.g. a temperature sensitive mutant over-expressed''': The gene expression has been significantly increased by engineering, e.g. by replacing the normal gene promoter with one that overexpresses the gene. *under-expressed''': The gene expression has been significantly decreased by engineering, e.g. by replacing the normal gene promoter with one that underexpresses the gene.

Discussion: These seem to be overlap somewhat with the 'experimental preparation' tree, but together, they mean something different than any existing branch. E.g. expression level overlaps with over-expressed. But are knock-out or hypomorph terms useful for physical interactions?

Thanks, Gary

Reported by: gbader

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Logged In: YES user_id=653048

Approved at the PSI meeting in Washington and terms added see parent term MI:0787, 'genetic experimental form'

Original comment by: luisa_montecchi

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• status: open --> closed

Original comment by: luisa_montecchi