Hello,
I am currelty running insilicoseq_1.5.3 to generate simulated reads from the mm39 mouse genome. I am running the program using the coverage_file option with a 30 coverage in chr1-19 and once reads are generated and mapped back to the mm39 reference genome the average coverage is significantly lower in chr19 compared to those of the other chromosomes.
overall coverage ends up being 29x and the coverage in chr19 is 3x
Hello, I am currelty running insilicoseq_1.5.3 to generate simulated reads from the mm39 mouse genome. I am running the program using the coverage_file option with a 30 coverage in chr1-19 and once reads are generated and mapped back to the mm39 reference genome the average coverage is significantly lower in chr19 compared to those of the other chromosomes. overall coverage ends up being 29x and the coverage in chr19 is 3x
have you seen this issue before?