Open Naturalist1986 opened 1 month ago
Hi!
Are all your contig names unique across draft genomes? InSilicoSeq currently uses the contig names as read headers.
Hi,
Yes, these are downloaded from NCBI Refseq. A portion of the genome contigs is missing from the generated fastqs, even though it is listed in the abundance file.
Hi,
I'm using this command to generate metagenomes from a set of genome fasta files:
iss generate --draft (list of *.fna files) --output --model nextseq --coverage uniform -p 128 -n 50M
Although the abundance file shows it uses 10 genomes for example, when I count the reads in the fastqs I only see 5-6 genomes.