Closed denvercal1234GitHub closed 1 year ago
IMHO PeacoQC is the right tool to use. So, you should address your issue to the github of PeacoQC. Issues section of github repositories mainly aims to solve a bug. If you are asking a general question, especially if it could be solved in different ways (PeacoQC, a loop on the FCS files...), you should ask it on the support forum of Bioconductor. Best.
Thank you, Sam. For anyone having the same Q, it was solved in PeacoQC issue as referenced above.
Hi there,
I hope to use the package to perform clustering of my spectral flow data, but I would like to first remove all events across all FCS files that have intensity greater than 2x10^6 for all markers, because they skewed my clusters.
My input = compensated, untransformed FCS.
I tried to use
PeacoQC::RemoveMargins()
as below (also in https://github.com/saeyslab/PeacoQC/issues/19), but it has not worked.Does
CATALYST
have some function to do this?Thank you for your help!
Error: