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Hendricks-Laboratory / OT2Control

software to provide detailed control of Opentrons OT2 robot
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Tip goes a little too deep when mixing full 15 mL tubes #52

Closed Hendricks-Laboratory closed 1 month ago

Hendricks-Laboratory commented 2 years ago

During mixing a 15/50 mL tube that's mostly full, the maximum depth the tip should go down is based on the length of the tip (so that we only submerge the tip and not the pipette itself). It seems like this is working, but it's going just a little too deep so it's just submerging past the tip/touching the pipette. I think 5 mm less deep/higher would solve the problem and then I can test again. Overall mixes are looking really good though!

Hendricks-Laboratory commented 2 years ago
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If we change all of these 55s to 50s I think we'd probably be okay!

Hendricks-Laboratory commented 2 years ago

The tip is still going too far in. I think we need to change the 55 on line 459 to 50 and try that, but then we might need to adjust both to something even smaller (although the tips are 59-60mm long, so I don't know why the 55 isn't working)

harveymk commented 2 years ago

Did this change!

mphendricks commented 2 years ago

I'm going to leave this open until it's tested

mphendricks commented 2 years ago

This is just barely good enough, with surface tension it's not going above the tip, but with a different solution/temperature/luck, it probably would touch. Let's change both of these 50s to 48 and I think we'll be good!