The meaning of sensitivity

[yrl1120]

Hello, I have a question about the eSRRF parameter, the change of sensitivity value means that something has changed in the calculation, is it the same as axes in ring in SRRF？

[yrl1120]

If I want to handle the same stack with the same parameters, how can sensitivity be represented in SRRF? Or is it mainly RADIUS that affects the final result in SRRF, and other parameters can be selected by default？

[HannahSHeil]

Hi, the eSRRF Sensitivity and SRRF Axis parameters are not comparable. The eSRRF sensitivity allows to fine-tune the PSF sharpening power and a contrast enhancement of the RGC (Radius Gradient Convergence) is performed by applying the user defined Sensitivity S value as a power to the RGC map. This operation was not integrated in the original SRRF application. This means it is not really possible to use the same parameters for eSRRF and SRRF. If you want to compare the performance of both, I would recommend to apply a parameter sweep and pick parameter sets that yield the best results in each case. As the parameter sweep is not integrated in SRRF you'll need to go for a makro to batch process FRC and RSP map calculations. Does this answer your questions? Best, Hannah

[yrl1120]

Thank you very much for your answer, this solved my confusion and I still have some questions to ask: how do you get FRC and RSP maps using SRRF, and in which plugin the makro you mentioned is?

[yrl1120]

I've solved the first question, but I'd like to ask you how to use Macro to batch FRC and RSP mapping calculations, and I would appreciate it if you could answer.

[HannahSHeil]

Hi, you'll need the NanoJ-Squirrel plugin. I'll just roughly sketch the steps, for FRC

• you need to split the stack in odd and even frames (run("Split image sequence into odd and even frames");)
• run SRRF on each substack: run("SRRF Analysis", "ring="+R[k]+" radiality_magnification="+M+" axes="+A[t]+" frames_per_time-point="+NfrEven+" start=0 end=0 max=100 preferred=0");
• calculate FRC resolution estimate to get mean FRC resolution for each parameter set: run("Calculate FRC-Map", "blocks=20 pixel="+pixelSize);

for RSP you only need a single WF image and a stack of all SRRF reconstructions, based on that you can calculate the RSP for each with Plugins > NanoJ-Squirrel > Calculate Error-Map, RSE and RSP UNICORN optimiser (beta)

Best, Hannah

[yrl1120]

Thank you very much for your answer, if i want to compare the reconstruction performance of SRRF and ESRRF, is there a corresponding relationship between the three temporal analysis methods of ESRRF and the three temporal analysis methods of SRRF, or choose the time analysis method with the best performance respectively for comparison?