Hoohm / CITE-seq-Count

A tool that allows to get UMI counts from a single cell protein assay
https://hoohm.github.io/CITE-seq-Count/
MIT License
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Gene and Antibody both have a pair of fastq files, how to run CITE-seq-Count? #189

Closed edceeyuchen closed 9 months ago

edceeyuchen commented 9 months ago

My files are below: Ab_C0042-25CN_S0_L001_I1_001.fastq.gz Ab_C0042-25CN_S0_L001_R1_001.fastq.gz Ab_C0042-25CN_S0_L001_R2_001.fastq.gz GEX_C0042-25CN_S0_L001_I1_001.fastq.gz GEX-C0042-25CN_S0_L001_R1_001.fastq.gz GEX-C0042-25CN_S0_L001_R2_001.fastq.gz

luostrowski commented 2 months ago

Were you able to figure this out?

Hoohm commented 1 month ago

Hello, CITE-seq-Count only runs on antibody fastq files, you need to merge them back using the cell barcode later after preprocessing.

Hope this helps.