Less detected lncRNA

[YOU-k]

Hi, I am noticing that less lncRNA are detected with dropSeqPipe. only 22 were found while with other tools using STAR I got much more, about 2k. I specified: META: species: mus_musculus: build: 38 release: 99 reference-directory: reference gtf_biotypes: gtf_biotypes.yaml

and in the gtf_biotypes.yaml:

• 3prime_overlapping_ncRNA
• antisense
• bidirectional_promoter_lncRNA
• IG_C_gene
• IG_C_pseudogene
• IG_D_gene
• IG_J_gene
• IG_J_pseudogene
• IG_pseudogene
• IG_V_gene
• IG_V_pseudogene
• lincRNA
• macro_lncRNA
• miRNA
• misc_RNA
• Mt_rRNA
• Mt_tRNA
• non_coding...... were included. What might be the reason for this? Thanks!

[YOU-k]

Sorry the problem is fixed. I just found out dropSeqPipe uses transcript biotypes.