Open MarleyCodes opened 5 years ago
Could you please provide an already to use example to run for testing with a fully filled yaml template example? It would be very helpful. I'm having the following issue. It's weird because I'm pretty sure that the fastq.gz files are there but the message say they aren't. I even use the full path.
SystemExit: No sample files found in the ~/sc/dropSeqPipe/data/fastq_slides/ directory.
Please check that the path for the raw data is set properly in config.yaml
This is the whole config.yaml file.
CONTACT:
email:
person:
LOCAL:
temp-directory: .tmp
memory: 4g
raw_data: ~/sc/dropSeqPipe/data/fastq_slides/
results: results
META:
species:
mus_musculus:
build: 38
release: 1
ratio: 0.2
reference-directory: ref
gtf_biotypes: gtf_biotypes.yaml
FILTER:
barcode-whitelist: ''
5-prime-smart-adapter: ''
cell-barcode:
start: 1
end: 12
UMI-barcode:
start: 13
end: 21
cutadapt:
adapters-file: NexteraPE-PE.fa
R1:
quality-filter: 20
maximum-Ns: 1
extra-params: ''
R2:
quality-filter: 20
minimum-adapters-overlap: 6
minimum-length: 15
extra-params: ''
MAPPING:
STAR:
genomeChrBinNbits: 18
outFilterMismatchNmax: 10
outFilterMismatchNoverLmax: 0.3
outFilterMismatchNoverReadLmax: 1
outFilterMatchNmin: 0
outFilterMatchNminOverLread: 0.66
outFilterScoreMinOverLread: 0.66
EXTRACTION:
LOCUS:
- CODING
- UTR
strand-strategy: SENSE
UMI-edit-distance: 1
minimum-counts-per-UMI: 0
DEBUG: False
Again a complete example would be great, maybe with a tiny number of fastq sequences to be able to run easily. Thanks for the tool ;).
Hello @caramirezal
I would not use relative paths at all. We sometimes get odd behaviors with those.
There is a test data in the .test folder. You can download this by using this command: git submodule update --init
Hi,
I'm having the same issue as @caramirezal I have .fastq.gz files in the data folder, but I still get the "no samples found" error. I tried the full paths in both the snakemake call and the yaml, but to no success.
I extracted the pipeline from the 0.4 tar.gz archive.
Any suggestions?
Thanks!
The pipeline does not seem to create the tmp files of the scripts within the batch/.snakemake/scripts directory. Which results in the crash of the following command:
source /home/mye/miniconda2/bin/activate '/data/sag/2019/MYE_18020068_2019_SingleCellTomBras/dropSeqPipe/batch1/.snakemake/conda/f854f536'; set -euo pipefail; Rscript --vanilla /data/sag/2019/MYE_18020068_2019_SingleCellTomBras/dropSeqPipe/batch1/.snakemake/scripts/tmp5wipn0w_.plot_violine.R'
With error:
Fatal error: cannot open file '/data/sag/2019/MYE_18020068_2019_SingleCellTomBras/dropSeqPipe/batch1/.snakemake/scripts/tmp5wipn0w_.plot_violine.R': No such file or directory
Is something going wrong with rights or directories? Any clues would be greatly appreciated.
config.yaml: