Project Completion QA Checklist: Multiplexed scRNA-seq with barcoded antibodies

[jychien]

Project UUID: f81efc03-9f56-4354-aabb-6ce819c3d414 Project Title: Cell hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics Project Short Name: Multiplexed scRNA-seq with barcoded antibodies Submission UUID: 6931d821-9cd2-4ae1-acf7-62cd3b90082e Environment: Production

• [ ] Process QA of bundles
• [ ] Metadata tsv validation: Run metadatatsv_validator.py

[jychien]

Process QA found the following issue:

1. There is no collection protocol. PBMC was obtained from AllCells.

[jychien]

2. The experiment was pooled at sequence file, where 1 file mapped to eight cell suspensions. sequence_file.library_prep_id field is not on the Sequence file tab. It is ambiguous as to whether it was 1 cDNA library that associated with each set of sequence files. Other pooled experiments (HPSI human cerebral organoids) was pooled at the Cell suspension tab, and each sequence file is associated with 1 pooled cell suspension,.

[jychien]

3. Should have antibody information and at least ADT mapping to cell barcode. Could also have additional cell metadata, such as singlet vs multiplet.