Project Completion QA Checklist: Single cell RNAseq characterization of cell types produced over time in an in vitro model of human inhibitory interneuron differentiation.

[jychien]

Project UUID: 2043c65a-1cf8-4828-a656-9e247d4e64f1 Project Title: Single-cell RNA-seq analysis throughout a 125-day differentiation protocol that converted H1 human embryonic stem cells to a variety of ventrally-derived cell types. Project Short Name: Single cell RNAseq characterization of cell types produced over time in an in vitro model of human inhibitory interneuron differentiation. Submission UUID: 0948a727-228f-4cfc-857e-6243c6aed08d Environment: Production

• [ ] Process QA of bundles
• [ ] Metadata tsv validation: Run metadatatsv_validator.py

[jychien]

Process QA found the following issue:

1. Project is missing collection protocol (Slack conversation). Not sure if this will require re-ingest to add a protocol. Has been added to hca-data-wrangling/issues/349
2. The 'Cell line' tab does not have any associated protocols, but instead has publication doi and url. Is this consistent representation between projects?
3. The Differentiation protocol 'P-GSE93593-1' describes process of constructing hESC cell lines and their neural differentiation. This protocol is listed as in the 'Cell suspension' tab, but would make more sense in the 'Cell line' tab.

[jychien]

Also found:

• cell_line_WAe001-A is a cell line without files that is not being displayed on the browser samples tab: