sbesson
commented
5 months ago Introspecting the content of the TIFF files
[sbesson@test122-omeroreadwrite ~]$ tiffinfo /data/OMERO/ManagedRepository/demo_2/Blitz-0-Ice.ThreadPool.Server-13/2021-06/05/12-54-52.237/141893_A_1_1.tif
=== TIFF directory 0 ===
TIFF Directory at offset 0x18c3614 (25966100)
Subfile Type: (0 = 0x0)
Image Width: 2942 Image Length: 2942 Image Depth: 1
Bits/Sample: 8
Compression Scheme: None
Photometric Interpretation: RGB color
Samples/Pixel: 3
Rows/Strip: 1
Planar Configuration: single image plane
ImageDescription: Aperio Image Library v8.2.43
81713x37331 [70791,733 2942x2942] (0x0) RAW;Scan
{composite image: 41 files}|AppMag = 20
|StripeWidth = 2032
|ScanScope ID = SS1644
|Filename = 156866
|Title =
|Date = 10/10/13
|Time = 09:14:17
|User = 85966804-1588-42c3-bd9e-88ca3cfca814
|Acquisition Bit Depth = 8
|MPP = 0.4970
|OriginalWidth = 81713|Originalheight = 37331Fundamentally we are dealing with original SVS files that have been post-processed (cropped and resolutions removed) with the original Aperio metadata preserved.
The structure conflicts with the assumptions made in https://github.com/ome/bioformats/blob/7ca9980651a5e776cd79ae4bb8f558eaa27bbb27/components/formats-gpl/src/loci/formats/in/SVSReader.java#L646-L648 to remove the thumbnail from the pyramidal levels. And disabling this logic via the reader option suffices to initialize the reader
[sbesson@test122-omeroreadwrite ~]$ ./bftools-7.3.0/showinf -nopix /data/OMERO/ManagedRepository/demo_2/Blitz-0-Ice.ThreadPool.Server-13/2021-06/05/12-54-52.237/141893_A_1_1.tif
Checking file format [Aperio SVS]
Initializing reader
SVSReader initializing /data/OMERO/ManagedRepository/demo_2/Blitz-0-Ice.ThreadPool.Server-13/2021-06/05/12-54-52.237/141893_A_1_1.tif
Reading IFDs
Populating metadata
Populating OME metadata
Exception in thread "main" java.lang.IllegalArgumentException: Invalid resolution: 0
at loci.formats.CoreMetadataList.flattenedIndex(CoreMetadataList.java:178)
at loci.formats.SubResolutionFormatReader.getSeries(SubResolutionFormatReader.java:145)
at loci.formats.MetadataTools.populatePixels(MetadataTools.java:135)
at loci.formats.MetadataTools.populatePixels(MetadataTools.java:116)
at loci.formats.in.BaseTiffReader.initMetadataStore(BaseTiffReader.java:426)
at loci.formats.in.SVSReader.initMetadataStore(SVSReader.java:669)
at loci.formats.in.BaseTiffReader.initMetadata(BaseTiffReader.java:99)
at loci.formats.in.BaseTiffReader.initFile(BaseTiffReader.java:610)
at loci.formats.FormatReader.setId(FormatReader.java:1480)
at loci.formats.ImageReader.setId(ImageReader.java:865)
at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:692)
at loci.formats.tools.ImageInfo.testRead(ImageInfo.java:1043)
at loci.formats.tools.ImageInfo.main(ImageInfo.java:1129)
[sbesson@test122-omeroreadwrite ~]$ ./bftools-7.3.0/showinf -nopix /data/OMERO/ManagedRepository/demo_2/Blitz-0-Ice.ThreadPool.Server-13/2021-06/05/12-54-52.237/141893_A_1_1.tif -option svs.remove_thumbnail false
Checking file format [Aperio SVS]
Initializing reader
SVSReader initializing /data/OMERO/ManagedRepository/demo_2/Blitz-0-Ice.ThreadPool.Server-13/2021-06/05/12-54-52.237/141893_A_1_1.tif
Reading IFDs
Populating metadata
Populating OME metadata
Initialization took 0.154s
Reading core metadata
filename = /data/OMERO/ManagedRepository/demo_2/Blitz-0-Ice.ThreadPool.Server-13/2021-06/05/12-54-52.237/141893_A_1_1.tif
Series count = 1
Series #0 :
Image count = 1
RGB = true (3)
Interleaved = false
Indexed = false (true color)
Width = 2942
Height = 2942
SizeZ = 1
SizeT = 1
SizeC = 3 (effectively 1)
Tile size = 2942 x 1
Thumbnail size = 128 x 128
Endianness = intel (little)
Dimension order = XYCZT (uncertain)
Pixel type = uint8
Valid bits per pixel = 8
Metadata complete = true
Thumbnail series = false
-----
Plane #0 <=> Z 0, C 0, T 0
Reading global metadata
BitsPerSample: 8
Comment: {composite image: 41 files}
Compression: Uncompressed
ImageLength: 2942
ImageWidth: 2942
MetaDataPhotometricInterpretation: RGB
NewSubfileType: 0
NumberOfChannels: 3
PhotometricInterpretation: RGB
PlanarConfiguration: Chunky
SamplesPerPixel: 3
Reading metadata
Acquisition Bit Depth: 8
AppMag: 20
Date: 10/10/13
Filename: 156866
MPP: 0.4970
OriginalWidth: 81713
Originalheight: 37331
ScanScope ID: SS1644
StripeWidth: 2032
Time: 09:14:17
User: 85966804-1588-42c3-bd9e-88ca3cfca814Thinking of solutions, I think of 2 possible routes:
- either make the thumbnail removal more defensive e.g. add a check for files with a single IFD
- or tighten the
SVSReader.isThisTypecheck to look for tiled images in the first IFD similar to OpenSlide /cc @melissalinkert
Reported at https://github.com/IDR/idr-metadata/issues/696#issuecomment-2191691918
ResourceErrors from idr0043, affecting Datasets from e.g 9101 -> 14734: E.g. viewing http://localhost:1080/webclient/img_detail/13004862/ Gives:
Stack trace
``` 2024-06-26 13:20:46,228 DEBUG [ loci.formats.Memoizer] (.Server-15) start[1719408046201] time[26] tag[loci.formats.Memoizer.setId] 2024-06-26 13:20:46,228 ERROR [ ome.io.bioformats.BfPixelBuffer] (.Server-15) Failed to instantiate BfPixelsWrapper with /data/OMERO/ManagedRepository/demo_2/Blitz-0-Ice.ThreadPool.Server-13/2021-06/05/12-54-52.237/141893_A_1_1.tif 2024-06-26 13:20:46,228 ERROR [ ome.io.nio.PixelsService] (.Server-15) Error instantiating pixel buffer: /data/OMERO/ManagedRepository/demo_2/Blitz-0-Ice.ThreadPool.Server-13/2021-06/05/12-54-52.237/141893_A_1_1.tif java.lang.RuntimeException: java.lang.IndexOutOfBoundsException: Index 0 out of bounds for length 0 at ome.io.bioformats.BfPixelBuffer.reader(BfPixelBuffer.java:79) at ome.io.bioformats.BfPixelBuffer.setSeries(BfPixelBuffer.java:124) at ome.io.nio.PixelsService.createBfPixelBuffer(PixelsService.java:898) at ome.io.nio.PixelsService._getPixelBuffer(PixelsService.java:653) at ome.io.nio.PixelsService.getPixelBuffer(PixelsService.java:571) at ome.services.RenderingBean$12.doWork(RenderingBean.java:2205) at jdk.internal.reflect.GeneratedMethodAccessor319.invoke(Unknown Source) at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.base/java.lang.reflect.Method.invoke(Method.java:566) at org.springframework.aop.support.AopUtils.invokeJoinpointUsingReflection(AopUtils.java:333) at org.springframework.aop.framework.ReflectiveMethodInvocation.invokeJoinpoint(ReflectiveMethodInvocation.java:190) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:157) at ome.services.util.Executor$Impl$Interceptor.invoke(Executor.java:568) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at ome.security.basic.EventHandler.invoke(EventHandler.java:154) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at org.springframework.orm.hibernate3.HibernateInterceptor.invoke(HibernateInterceptor.java:119) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at org.springframework.transaction.interceptor.TransactionInterceptor$1.proceedWithInvocation(TransactionInterceptor.java:99) at org.springframework.transaction.interceptor.TransactionAspectSupport.invokeWithinTransaction(TransactionAspectSupport.java:282) at org.springframework.transaction.interceptor.TransactionInterceptor.invoke(TransactionInterceptor.java:96) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at ome.tools.hibernate.ProxyCleanupFilter$Interceptor.invoke(ProxyCleanupFilter.java:249) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at ome.services.util.ServiceHandler.invoke(ServiceHandler.java:121) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at org.springframework.aop.framework.JdkDynamicAopProxy.invoke(JdkDynamicAopProxy.java:213) at com.sun.proxy.$Proxy101.doWork(Unknown Source) at ome.services.util.Executor$Impl.execute(Executor.java:447) at ome.services.util.Executor$Impl.execute(Executor.java:392) at ome.services.RenderingBean.getPixelBuffer(RenderingBean.java:2202) at ome.services.RenderingBean.load(RenderingBean.java:417) at jdk.internal.reflect.GeneratedMethodAccessor1342.invoke(Unknown Source) at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.base/java.lang.reflect.Method.invoke(Method.java:566) at org.springframework.aop.support.AopUtils.invokeJoinpointUsingReflection(AopUtils.java:333) at org.springframework.aop.framework.ReflectiveMethodInvocation.invokeJoinpoint(ReflectiveMethodInvocation.java:190) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:157) at ome.services.util.ServiceHandler.invoke(ServiceHandler.java:121) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at org.springframework.aop.framework.JdkDynamicAopProxy.invoke(JdkDynamicAopProxy.java:213) at com.sun.proxy.$Proxy122.load(Unknown Source) at jdk.internal.reflect.GeneratedMethodAccessor1342.invoke(Unknown Source) at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.base/java.lang.reflect.Method.invoke(Method.java:566) at org.springframework.aop.support.AopUtils.invokeJoinpointUsingReflection(AopUtils.java:333) at org.springframework.aop.framework.ReflectiveMethodInvocation.invokeJoinpoint(ReflectiveMethodInvocation.java:190) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:157) at ome.security.basic.BasicSecurityWiring.invoke(BasicSecurityWiring.java:93) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at ome.services.blitz.fire.AopContextInitializer.invoke(AopContextInitializer.java:43) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at org.springframework.aop.framework.JdkDynamicAopProxy.invoke(JdkDynamicAopProxy.java:213) at com.sun.proxy.$Proxy122.load(Unknown Source) at jdk.internal.reflect.GeneratedMethodAccessor1417.invoke(Unknown Source) at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.base/java.lang.reflect.Method.invoke(Method.java:566) at ome.services.blitz.util.IceMethodInvoker.invoke(IceMethodInvoker.java:172) at ome.services.throttling.Callback.run(Callback.java:56) at ome.services.throttling.InThreadThrottlingStrategy.callInvokerOnRawArgs(InThreadThrottlingStrategy.java:56) at ome.services.blitz.impl.AbstractAmdServant.callInvokerOnRawArgs(AbstractAmdServant.java:140) at ome.services.blitz.impl.RenderingEngineI.load_async(RenderingEngineI.java:316) at jdk.internal.reflect.GeneratedMethodAccessor1416.invoke(Unknown Source) at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.base/java.lang.reflect.Method.invoke(Method.java:566) at org.springframework.aop.support.AopUtils.invokeJoinpointUsingReflection(AopUtils.java:333) at org.springframework.aop.framework.ReflectiveMethodInvocation.invokeJoinpoint(ReflectiveMethodInvocation.java:190) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:157) at omero.cmd.CallContext.invoke(CallContext.java:85) at org.springframework.aop.framework.ReflectiveMethodInvocation.proceed(ReflectiveMethodInvocation.java:179) at org.springframework.aop.framework.JdkDynamicAopProxy.invoke(JdkDynamicAopProxy.java:213) at com.sun.proxy.$Proxy124.load_async(Unknown Source) at omero.api._RenderingEngineTie.load_async(_RenderingEngineTie.java:248) at omero.api._RenderingEngineDisp.___load(_RenderingEngineDisp.java:1223) at omero.api._RenderingEngineDisp.__dispatch(_RenderingEngineDisp.java:2405) at IceInternal.Incoming.invoke(Incoming.java:221) at Ice.ConnectionI.invokeAll(ConnectionI.java:2536) at Ice.ConnectionI.dispatch(ConnectionI.java:1145) at Ice.ConnectionI.message(ConnectionI.java:1056) at IceInternal.ThreadPool.run(ThreadPool.java:395) at IceInternal.ThreadPool.access$300(ThreadPool.java:12) at IceInternal.ThreadPool$EventHandlerThread.run(ThreadPool.java:832) at java.base/java.lang.Thread.run(Thread.java:829) Caused by: java.lang.IndexOutOfBoundsException: Index 0 out of bounds for length 0 at java.base/jdk.internal.util.Preconditions.outOfBounds(Preconditions.java:64) at java.base/jdk.internal.util.Preconditions.outOfBoundsCheckIndex(Preconditions.java:70) at java.base/jdk.internal.util.Preconditions.checkIndex(Preconditions.java:248) at java.base/java.util.Objects.checkIndex(Objects.java:374) at java.base/java.util.ArrayList.get(ArrayList.java:459) at loci.formats.MetadataList.get(MetadataList.java:121) at loci.formats.SubResolutionFormatReader.getCurrentCore(SubResolutionFormatReader.java:238) at loci.formats.FormatReader.getPixelType(FormatReader.java:735) at loci.formats.MetadataTools.populatePixels(MetadataTools.java:149) at loci.formats.MetadataTools.populatePixels(MetadataTools.java:116) at loci.formats.in.BaseTiffReader.initMetadataStore(BaseTiffReader.java:426) at loci.formats.in.SVSReader.initMetadataStore(SVSReader.java:669) at loci.formats.in.BaseTiffReader.initMetadata(BaseTiffReader.java:99) at loci.formats.in.BaseTiffReader.initFile(BaseTiffReader.java:610) at loci.formats.FormatReader.setId(FormatReader.java:1480) at loci.formats.ImageReader.setId(ImageReader.java:865) at ome.io.nio.PixelsService$3.setId(PixelsService.java:869) at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:692) at loci.formats.ChannelFiller.setId(ChannelFiller.java:258) at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:692) at loci.formats.ChannelSeparator.setId(ChannelSeparator.java:317) at loci.formats.ReaderWrapper.setId(ReaderWrapper.java:692) at loci.formats.Memoizer.setId(Memoizer.java:726) at ome.io.bioformats.BfPixelsWrapper.Seb: https://github.com/ome/bioformats/pull/4144