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IMB-Computational-Genomics-Lab / ascend

R package - Analysis of Single Cell Expression, Normalisation and Differential expression (ascend)
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Failed to convert em.set to SCESet or SingleCellExperiment #13

Closed MichaelPeibo closed 6 years ago

MichaelPeibo commented 6 years ago

hi,ascend team I got this error when I did conversion as your tutorial told: controls <- GetControls(em.set) sce.object <- ConvertToSCE(em.set, control.list = controls) sce.object

or

controls <- GetControls(em.set) sce.set <- ConvertToSCESet(em.set, control.list = controls)

but I got this error

Error in seq_len(ncol(assay)) : argument must be coercible to non-negative integer In addition: Warning messages: 1: 'newSCESet' is deprecated. Use 'SingleCellExperiment' instead. See help("Deprecated") 2: In seq_len(ncol(assay)) : first element used of 'length.out' argument

any suggestion?

MichaelPeibo commented 6 years ago

Somehow I fixed the issue, but i still got a problem when I did filtering in the scater workflow....