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Illumina / BeadArrayFiles

Python library to parse file formats related to Illumina bead arrays
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GTC file version 3 can't output B allele frequencies and logr #20

Open Liiiii2101 opened 4 years ago

Liiiii2101 commented 4 years ago

Thank you so much for making this repo. The B allele frequencies and logr values are important features for our studies but the version of our GTC files is 3, which means we cannot get those two features from your library. Do you know any other way to get these two features or the way to convert idat files to gtc version 4 or 5.

Thank you very much!

jjzieve commented 4 years ago

@Liiiii2101 You can genotype (i.e. IDAT->GTC conversion) via https://emea.support.illumina.com/array/array_software/illumina-array-analysis-platform.html which should create GTC version 5 with B Allele Frequencies and LogRRatios

Liiiii2101 commented 4 years ago

@Liiiii2101 You can genotype (i.e. IDAT->GTC conversion) via https://emea.support.illumina.com/array/array_software/illumina-array-analysis-platform.html which should create GTC version 5 with B Allele Frequencies and LogRRatios

Thank you! I used iaap to convert the idat files to gtcs, but there's another issue that the gtc files are incomplete. Do you know any methods to solve it?

Thank you very much!

jjzieve commented 4 years ago

I would contact illumina support directly (techsupport@illumina.com) for issues related to iaap, there may be a workaround.

jjzieve commented 3 years ago

@Liiiii2101 Was this ever resolved?

mikaelamo commented 2 years ago

I am having the same issue. Did any of you find a solution?

jjzieve commented 2 years ago

@mikaelamo Did you try running iaap? Did you get the same gtc incomplete error?

mikaelamo commented 2 years ago

@jjzieve The GTC files I have were created by using iaap, which is why I feel that it's kinda weird that they should be incomplete since iaap is supposed to be compatible with BeadArrayFiles. So far, I have only used the example code from the README.md, to see if I can get anything to work before deep diving but alas...

jjzieve commented 2 years ago

@mikaelamo Yes in general, iaap should be compatible. But because the original GTCs were version 3, I'd assume the product you're running is pretty old. So something untested/expected might be happening. Its hard to say for sure what's going on. I recommend the same path I recommended @Liiiii2101. To reach out to techsupport@illumina.com so we can open a support channel where we can take a look at your GTCs and/or IDATs and debug more closely. I don't remember if @Liiiii2101 ever reached out to support but it seems I asked about it and never got a response. Hopefully if we find out the root cause of your issue, the findings can be helpful to others here.