ERROR: Object reference not set to an instance of an object.
Nirvana (c) 2022 Illumina, Inc.
Stromberg, Roy, Platzer, Siddiqui, Ouyang, et al 3.18.0
Initialization Time Positions/s
Cache 00:00:01.4
ERROR: Object reference not set to an instance of an object.
Stack trace:
at VariantAnnotation.GenericScore.ScoreIndex.Read(Stream stream, Int32 dataFilePairId) in ~/Nirvana/VariantAnnotation/GenericScore/ScoreIndex.cs:line 154
at VariantAnnotation.GenericScore.ScoreReader.Read(Stream dataStream, Stream indexStream) in ~/Nirvana/VariantAnnotation/GenericScore/ScoreReader.cs:line 56
at Nirvana.ProviderUtilities.GetGsaProvider(AnnotationFiles files) in ~/Nirvana/Nirvana/ProviderUtilities.cs:line 87
at Nirvana.AnnotationResources..ctor(String refSequencePath, String inputCachePrefix, List1 saDirectoryPaths, List1 customAnnotations, String customStrTsvPath, Boolean disableRecomposition, Boolean forceMitochondrialAnnotation, Boolean useLegacyVids, PerformanceMetrics metrics) in ~/Nirvana/Nirvana/AnnotationResources.cs:line 69
at Nirvana.Nirvana.GetAnnotationResources() in ~/Nirvana/Nirvana/Nirvana.cs:line 61
at Nirvana.Nirvana.ProgramExecution() in ~/Nirvana/Nirvana/Nirvana.cs:line 38
at CommandLine.Builders.ConsoleAppErrors.Execute(Func`1 executeMethod) in ~/Nirvana/CommandLine/Builders/ConsoleAppBuilder.cs:line 162
I am running the test command:
dotnet bin/Release/netcoreapp3.1/Nirvana.dll \ -c Data/Cache/GRCh37/Both \ --sd Data/SupplementaryAnnotation/GRCh37 \ -r Data/References/Homo_sapiens.GRCh37.Nirvana.dat \ -i HiSeq.10000.vcf.gz \ -o HiSeq.10000
ERROR: Object reference not set to an instance of an object.
Nirvana (c) 2022 Illumina, Inc. Stromberg, Roy, Platzer, Siddiqui, Ouyang, et al 3.18.0
Initialization Time Positions/s
Cache 00:00:01.4
ERROR: Object reference not set to an instance of an object.
Stack trace: at VariantAnnotation.GenericScore.ScoreIndex.Read(Stream stream, Int32 dataFilePairId) in ~/Nirvana/VariantAnnotation/GenericScore/ScoreIndex.cs:line 154 at VariantAnnotation.GenericScore.ScoreReader.Read(Stream dataStream, Stream indexStream) in ~/Nirvana/VariantAnnotation/GenericScore/ScoreReader.cs:line 56 at Nirvana.ProviderUtilities.GetGsaProvider(AnnotationFiles files) in ~/Nirvana/Nirvana/ProviderUtilities.cs:line 87 at Nirvana.AnnotationResources..ctor(String refSequencePath, String inputCachePrefix, List
1 saDirectoryPaths, List
1 customAnnotations, String customStrTsvPath, Boolean disableRecomposition, Boolean forceMitochondrialAnnotation, Boolean useLegacyVids, PerformanceMetrics metrics) in ~/Nirvana/Nirvana/AnnotationResources.cs:line 69 at Nirvana.Nirvana.GetAnnotationResources() in ~/Nirvana/Nirvana/Nirvana.cs:line 61 at Nirvana.Nirvana.ProgramExecution() in ~/Nirvana/Nirvana/Nirvana.cs:line 38 at CommandLine.Builders.ConsoleAppErrors.Execute(Func`1 executeMethod) in ~/Nirvana/CommandLine/Builders/ConsoleAppBuilder.cs:line 162Can you help me out?
Thank you very much!