Pisces 5.2.9.23 is implemented in Illumina pipeline for Miseq to analyze Trusight panel.
It is not clear how DP/AD (format) was calculated in VCF file. We notices that DP is near the half of real depth, which indicated the stitching.
Could you explain the methodology used to calculate DP/AD. and how can we move to normal DP/AD format, that count each single read used in the genotyping?
Hi,
Pisces 5.2.9.23 is implemented in Illumina pipeline for Miseq to analyze Trusight panel.
It is not clear how DP/AD (format) was calculated in VCF file. We notices that DP is near the half of real depth, which indicated the stitching.
Could you explain the methodology used to calculate DP/AD. and how can we move to normal DP/AD format, that count each single read used in the genotyping?
Kind regards,
Ahmed