search

Illumina / isaac2

Aligner for sequencing data
Other
21 stars 4 forks source link

Base quality scores [0-63] supported only. #8

Closed zeus19900814 closed 9 years ago

zeus19900814 commented 9 years ago

Hi,

I was aligning a fastq file and I got the error "Invalid quality 72 found in ....... Base quality scores [0-63] supported only." Just realized that the fastq file quality score is Phred+64 encoded. Does Isaac only support Phred+33? Can I set some parameters to make it work with Phred+64?

Thanks in advance. Ivan

rpetrovski commented 9 years ago

No, iSAAC expects phred33 qualities from fastq.

Roman.