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ImperialCollegeLondon
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PombeTrack
Interface to permit the partially automated tracking of Schizosaccharomyces pombe cells from time-lapse data (especially ONIX data).
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Analysis functionality
#15
Closed
mountainpenguin
closed
5 years ago
mountainpenguin
commented
5 years ago
Needs:
[ ] nuclear outlining (editable)
[x] hull method from too_lazy_to_click (1.1 * stddev + mean)
[x] background subtraction (from wild-type)
[x] editing
[ ] setting correct nuclear channel
[x] illumination correction
[ ] timing information
[x] px to um information
[x] incorporation of wild-type cells from other experiments
[ ] combination of multiple experiments (e.g. replicates)
[x] retrieval of data:
[x] cell area
[x] time to mitosis
[x] total fluorescence (all channels)
[x] mean fluorescence (by cell area; all channels)
[x] nuclear fluorescence (all channels)
[x] mean nuclear fluorescence (by nuclear area; all channels)
[x] nuclear area
cell-specific data:
[x] growth rate
[x] interdivision time
[x] birth_area, division_area
[ ] generation number (observed)
mountainpenguin
commented
5 years ago
Superseded by #43
Needs: